However in Koshland suggested an Induced Fit hypothesis. However the colorimeter uses a filter to only let some wavelengths of light through or else it would let all the light through not showing distinctions between different solutions.
Hydrochloric acid is produced in the stomach to destroy harmful bacteria and this makes the enzymes in the stomach work best in its acidic conditions pH 2. This was discovered on the pH of 5. The pepsin in the stomach is a protease which works best at the acidic conditions of the stomach so if the rate of reaction increases in acidic conditions, it will determine the acid is made in the stomach and in the duodenum if it works best in alkaline conditions.
Using a water bath would keep the temperature constant but very difficult as keeping the temperature constant would mean keeping the solution in the water bath until the reaction is complete. The stop clock will be started as soon as the two solutions are mixed together in the testube and the solution in the testube will be poured into the cuvette which will be put into the colorimeter.
These theories helped explain what went on when the casein binded with the trypsin and this helps show that the casein was the right shape for the trypsin in my experiment.
There are two theories that show how enzymes work and are shown below. Carefully place a 1cm piece of photographic film in each notch on the splints. Temperature increases the rate of reaction as there is more kinetic energy given to the enzyme so it moves around faster increasing the chance of a successful enzyme-substrate collision.
Many other enzymes catalyse other types of reactions. An enzyme is a globular protein molecule which in a biochemical reaction as a catalyst. The anomaly could have been obtained due to many reasons.
They were written down and the average time was found out. Therefore a filter must be used and as the come in different colours letting only some wavelengths of light through so the most suitable was chosen for the experiment which was nm.
This hypothesis stated that the substrates were vaguely the same shape of the active site but when the enzyme is enters the active site, it moulds the shape of the active site to allow more efficient binding and catalysis.
Clean the test tubes and apparatus thoroughly. Any spillages will be mopped up and any broken glass reported and cleared up carefully and disposed in a special glass bin.
The reaction therefore would be monitored using a colorimeter that measures the amount of light going through the solution and the more light that is let through, the more the amount of substrate has been broken down.
There were no real noticeable anomalies that can be noted as outside the conventional result and thus there were no results outside the expected results.Trypsin Investigation Essay by KatieCutie, High School, 11th grade, A- March download word file, 4 pages download word file, 4 pages 2 votes5/5(2).
Free Essay: Investigation into the Digestion of Milk by Trypsin Background Knowledge ===== To investigate the effect of trypsin on milk a.
Enzyme activity can be affected by the change of pH, temperature We will write a custom essay sample on Any topic specifically for you For Only $/page.
In this investigation, the trypsin did not work best at acidic conditions and the rate of reaction was affected because active site (where the reaction happens on the enzyme) is. Investigation into the effects of trypsin on dried milk solution For this investigation we are only required to carry out the analysis and evaluation.
We formulated our own hypotheses. The teacher carried out the experiment and provided us with the results. Enzyme Investigation. Essay by manutdchick4eva, High School, 12th grade, October download word file, 12 pages, 1 reviews. Downloaded 78 times. Keywords Science Coursework Trypsin Investigation Aim I am going to prove that temperature effects the rate in which the enzyme Trypsin breaks down milk substrate into amino 4/5(1).
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